Phospho-Bcl-xL (Ser62) Rabbit Polyclonal Antibody

SKU: orb608144

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Description

Images & Validation

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Item 1 of 2

Tested Applications	ICC, IF, IHC-Fr, IHC-P
Dilution range	IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
Reactivity	Human, Rat
Predicted Reactivity	Bovine, Canine, Equine, Mouse, Porcine, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human Bcl-xL around the phosphorylation site of Ser62 (55-200/233aa)
Target	BCL2L1
Molecular Weight	26 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Apoptosis regulator Bcl X; BclX; Bcl-X; Bcl 2 like 1; Bcl 2 like 1 protein; Bcl xL; BCL X/L; BCL XL/S; Bcl xS; Bcl2 like1; BCL2-related gene; BCL2-related protein, long isoform, included; BCLXL, included; BCL2-related protein, short isoform, included; BCLXS, included; BCL2L; Bclx; DKFZp781P2092; MGC113803; MGC99998; B2CL1_HUMAN.

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Phospho-Bcl-xL (Ser62) Rabbit Polyclonal Antibody [orb312836]
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Phospho-Bcl-xL (Ser62) Rabbit Polyclonal Antibody (Biotin) [orb608122]
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Phospho-Bcl-xL (Ser62) Rabbit Polyclonal Antibody (HRP) [orb608134]
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Phospho-Bcl-xL (Ser62) Rabbit Polyclonal Antibody

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-Bcl-xL (Ser62)) polyclonal Antibody, Unconjugated (orb608144) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-Bcl-xL (Ser62)) Polyclonal Antibody, Unconjugated (orb608144) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

BCL2L1

UniProt

Q07817

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ICC

Immunocytochemistry

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