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Phospho-CD130/gp130 (Ser782) Rabbit Polyclonal Antibody
SKU: orb157259
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
| Reactivity | Human, Rabbit, Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Gallus, Mouse, Porcine |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthesised phosphopeptide derived from human gp130 around the phosphorylation site of Ser782 SE(p-S)TQ |
| Target | IL6ST |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−gp130(phospho S782); gp130(phospho Ser782); p-gp130(Ser782); Interleukin-6 receptor subunit beta; IL-6R-beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; CDw130; Oncostatin-M receptor subunit alpha; CD_antigen; CD130; GP130 RAPS; gp130 transducer chain; GP130-RAPS; IL6 ST; IL6R-beta; IL6ST.IL6R-beta; IL6RB_HUMAN; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB.
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Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-gp130 (Ser782)) Polyclonal Antibody, Unconjugated (orb157259) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-gp130 (Ser782)) Polyclonal Antibody, Unconjugated (orb157259) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
phosphopeptide, non phosphopeptide.
Sample: Lane 1: Human HepG2 cell Lysates, Lane 2: Human HUVEC cell Lysates, Primary: Anti-phospho-CD130gp130 (Ser782) (orb157259) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130kDa, Observed band size: 130kDa.
Quick Database Links
Gene Symbol
IL6ST
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Phospho-CD130/gp130 (Ser782) Rabbit Polyclonal Antibody (orb157259)
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