Phospho-CD19 (Tyr531) Rabbit Polyclonal Antibody

SKU: orb106064

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Description

Research Area

CD Markers, Cell Biology, Human Lineage-negative, Stem Cell, Stem Cell Research

Images & Validation

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Item 1 of 3

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat, Sheep

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human CD19 around the phosphorylation site of Tyr531 DS(p-Y)EN
Target	CD19
Molecular Weight	59 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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CD19_HUMAN; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD_antigen: CD19; B-lymphocyte surface antigen B4; Leu 12; Leu12;

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Phospho-CD19 (Tyr531) Rabbit Polyclonal Antibody

Blank control (blue line): HL60 cells (fixed with 70% methanol (Overnight at 4°C). Cells stained with Primary Antibody for 30 min at room temperature). Primary Antibody (green line): Rabbit Anti-CD19 antibody (orb106064), Dilution: 1 µg/10^6 cells. Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test.

phosphopeptide, non phosphopeptide.

Sample: Lane 1: Human Raji cell lysates, Lane 2: Human K562 cell lysates, Primary: Anti-Phospho-CD19 (Tyr531) (orb106064) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 59 kD, Observed band size: 105 kD.

Quick Database Links

Gene Symbol

CD19

UniProt

P15391

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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