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Phospho-CEBP alpha (Ser21) Rabbit Polyclonal Antibody

SKU: orb5840
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Description

Phospho-CEBP alpha (Ser21) Rabbit Polyclonal Antibody

Images & Validation

Tested ApplicationsICC
Dilution rangeICC/IF=1:50-200
ReactivityHuman
Predicted ReactivityBovine, Mouse, Porcine, Rabbit, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human CDBP alpha around the phosphorylation site of Ser21 LQ(p-S)PP
TargetCEBPA
Molecular Weight38 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

Apoptotic cysteine protease; Apoptotic protease Mch 5; C/EBP alpha; CAP4; Caspase 8 precursor; CCAAT Enhancer Binding Protein alpha; CEBP; CEBP A; CEBP alpha; CEBPA; FADD homologous ICE/CED 3 like protease; FADD like ICE; FLICE; ICE like apoptotic protease 5; ICE8; MACH; MCH5; MORT1 associated CED 3 homolog; CEBPA_HUMAN.

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Phospho-CEBP alpha (Ser21) Rabbit Polyclonal Antibody

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-CEBP alpha (Ser21)) polyclonal Antibody, Unconjugated (orb5840) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Phospho-CEBP alpha (Ser21) Rabbit Polyclonal Antibody

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-CEBP alpha (Ser21)) polyclonal Antibody, Unconjugated (orb5840) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

UniProt Details

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Protocol Information

ICC
Immunocytochemistry
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Phospho-CEBP alpha (Ser21) Rabbit Polyclonal Antibody (orb5840)

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Available Sizes

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50 μl
¥ 3,120.00
100 μl
¥ 5,070.00
200 μl
¥ 6,630.00
DispatchUsually dispatched within 5-10 working days
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