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Phospho-EphB1+EphB2 (Tyr594) Rabbit Polyclonal Antibody

SKU: orb183490

Description

Phospho-EphB1+EphB2 (Tyr594) Rabbit Polyclonal Antibody

Research Area

Axonal Guidance Proteins, Cell Biology, Eph Eeceptors, Kinases, Neuroscience, Receptor Tyrosne Kinases, Tyrosine Kinase Signaling Pathway

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityHuman
Predicted ReactivityMouse, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthesised phosphopeptide derived from human EphB1+EphB2 around the phosphorylation site of Tyr594 KI(p-Y)ID
TargetEPHB1
Molecular Weight110 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

Eph receptor B1+Eph receptor B2(phospho Y594); p-Eph receptor B1+Eph receptor B2(phospho Y594); Eph receptor B1+Eph receptor B2; EPH B1; EPH B2; Eph tyrosine kinase 2; Eph tyrosine kinase 3; EPHB1; EPHB2; Ephrin receptor EphB1; Ephrin receptor EphB2; Ephrin type B receptor 1; Ephrin type B receptor 2; EPHT 2; EPHT 3; ERK; Hek 5; Hek 6; Tyrosine protein kinase receptor EPH2; Tyrosine protein kinase receptor EPH3.

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Phospho-EphB1+EphB2 (Tyr594) Rabbit Polyclonal Antibody

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-EPHB1 Polyclonal Antibody, Unconjugated (orb183490) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

UniProt Details

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

Phospho-EphB1+EphB2 (Tyr594) Rabbit Polyclonal Antibody (orb183490)

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50 μl
¥ 3,250.00
100 μl
¥ 5,070.00
200 μl
¥ 6,110.00
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