Phospho-Estrogen Receptor alpha (Ser104) Rabbit Polyclonal Antibody

SKU: orb183442

Description

Images & Validation

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Item 1 of 1

Tested Applications	IF, IHC-Fr, IHC-P
Dilution range	IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Rat
Predicted Reactivity	Bovine, Equine, Human, Mouse, Porcine

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human ER alpha around the phosphorylation site of Ser104 SV(p-S)P
Target	ESR1
Molecular Weight	66 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Estrogen Receptor alpha(phospho S104); ER(phospho S118); p-ER(phospho S118); ER alpha(phospho-Ser118); ER alpha(phospho-S104); phospho-ER alpha(S104); ; phospho-ER alpha(Ser104); p-ER alpha(Ser104); p-ER alpha(S104); phospho-Estrogen Receptor alpha(S104); phospho-Estrogen Receptor alpha(S104); Estradiol receptor; Estrogen receptor alpha; Estradiol Receptor-alpha; Estrogen Receptor 1; Atherosclerosis, susceptibility to, included; DKFZp686N23123; ER alpha; ER; ER-alpha; ERalpha; ER[a]; Era; ESR; ESR1; ESR1_HUMAN; ESR2; ESRA; Estr; Estrogen receptor 1(alpha); Estrogen resistance, included; HDL cholesterol, augmented response of, to hormone replacement, included; Myocardial infarction, susceptibility to, included; NR3A1; Nuclear receptor subfamily 3 group A member 1; OTTHUMP00000017718; OTTHUMP00000017719; RNESTROR.

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Phospho-Estrogen Receptor alpha (Ser104) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat uterus tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ESR1) Polyclonal Antibody, Unconjugated (orb183442) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

ESR1

UniProt

P03372

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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