Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthesised phosphopeptide derived from human APE around the phosphorylation site of Ser1417 QK(p-S)LT
Target	CCDC88A
Molecular Weight	206 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Girdin(phospho S1417); p-APE(Ser1417); GRDN_HUMAN; CCDC88A; APE; GRDN; KIAA1212; Akt phosphorylation enhancer(APE); Coiled-coil domain-containing protein 88A; G alpha-interacting vesicle-associated protein(GIV); Girders of actin filament; Hook-related protein 1(HkRP1);

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Phospho-Girdin (Ser1417) Rabbit Polyclonal Antibody

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-phospho-APE (Ser1417) antibody (orb1726), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min atroom temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Hela Cell (Human) Lysate at 40 ug, Primary: Anti-phospho-APE (Ser1417) (orb1726) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 206 kD, Observed band size: 206 kD.

Quick Database Links

Gene Symbol

CCDC88A

UniProt

Q3V6T2

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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