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Phospho-GSK-3 Beta (Ser21+Ser29) Rabbit Polyclonal Antibody
SKU: orb5356
Featured
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Ser21+Ser29 QP(p-S)AFGSMKV(p-S)RD |
| Target | GSK3B |
| Molecular Weight | 46 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−GSK3B(phospho-Ser21+Ser29); GSK3B(phospho-S21+S29); p-GSK-3 beta(Ser21+Ser29); p-GSK-3 beta(S21+S29); Glycogen synthase kinase-3 beta; glycogen synthase kinase 3 beta; GSK3β; GSK 3 beta; GSK 3B; GSK3B; GSK3B protein; GSK3beta isoform; GSK3 beta; Serine/threonine-protein kinase GSK3B; GSK3B_HUMAN.
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A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-GSK-3 Beta (Ser21+Ser29)) polyclonal Antibody, Unconjugated (orb5356) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (phospho-GSK-3 Beta (Ser21+Ser29)) Polyclonal Antibody, Unconjugated (orb5356) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (phospho-GSK-3 Beta (Ser21+Ser29)) Polyclonal Antibody, Unconjugated (orb5356) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GSK-3 Beta (Ser21+Ser29)) Polyclonal Antibody, Unconjugated (orb5356) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Muscle (Mouse) Lysate at 40 ug, Lane 2: Liver (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Mouse) Lysate at 40 ug, Lane 4: Pancreas (Mouse) Lysate at 40 ug, Lane 5: Thymus (Mouse) Lysate at 40 ug, Lane 6: Muscle (Rat) Lysate at 40 ug, Lane 7: Cerebrum (Rat) Lysate at 40 ug, Lane 8: Pancreas (Rat) Lysate at 40 ug, Lane 9: Thymus (Rat) Lysate at 40 ug, Lane 10: Hela (Human) Cell Lysate at 30 ug, Lane 11: MCF-7 (Human) Cell Lysate at 30 ug, Lane 12: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-GSK-3 Beta (Ser21+Ser29) (orb5356) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 47 kD.
Quick Database Links
Gene Symbol
GSK3B
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
Phospho-GSK-3 Beta (Ser21+Ser29) Rabbit Polyclonal Antibody (orb5356)
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