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Phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) Rabbit Polyclonal Antibody
SKU: orb783420
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Description
Images & Validation
−Item 1 of 3
| Tested Applications | WB |
|---|---|
| Dilution range | WB=1:500-2000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Tyr216 VS(p-Y)IC |
| Target | GSK3B |
| Molecular Weight | 47/51 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−GSK3B(phospho-Tyr216); GSK3B(phospho-Y216); p-GSK-3 beta(Tyr216); p-GSK-3 beta(Y216); Glycogen synthase kinase 3 beta; GSK 3 beta; GSK 3B; GSK3B; GSK3B protein; GSK3beta isoform; GSK3 beta; Glycogen synthase kinase-3 beta; GSK-3 beta; Glycogen synthase kinase-3 alpha; GSK3A; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A_HUMAN; GSK3B_HUMAN.
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Positive control: RSC96, Isotype Control Antibody: Rabbit IgG, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody catalog number: orb783420, dilution: 3 µl in 100 µl 1X PBS containing 0.5% BSA.
Sample: Lane 1: Brain (Mouse) Lysate at 30 ug, Lane 2: Testis (Mouse) Lysate at 30 ug, Primary: Anti-phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) (orb783420) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 47/51 kD, Observed band size: 50 kD.
Sample: Lane 1: Muscle (Mouse) Lysate at 40 ug, Lane 2: Liver (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Mouse) Lysate at 40 ug, Lane 4: Pancreas (Mouse) Lysate at 40 ug, Lane 5: Thymus (Mouse) Lysate at 40 ug, Lane 6: Muscle (Rat) Lysate at 40 ug, Lane 7: Liver (Rat) Lysate at 40 ug, Lane 8: Cerebrum (Rat) Lysate at 40 ug, Lane 9: Pancreas (Rat) Lysate at 40 ug, Lane 10: Thymus (Rat) Lysate at 40 ug, Lane 11: MCF-7 (Human) Cell Lysate at 30 ug, Lane 12: A431 (Human) Cell Lysate at 30 ug, Lane 13: U2os (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) (orb783420) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47'51 kD, Observed band size: 47 kD.
Quick Database Links
Gene Symbol
GSK3B
UniProt
UniProt Details
− No UniProt data available
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Phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) Rabbit Polyclonal Antibody (orb783420)
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