Phospho-GSK3 Beta (Tyr216) Rabbit Polyclonal Antibody

SKU: orb5359

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Description

Images & Validation

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Item 1 of 3

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit, Sheep

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Tyr216 VS(p-Y)IC
Target	GSK3B
Molecular Weight	47 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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GSK3 beta(phospho Y216); p-GSK3 beta(phospho Y216); GSK3B(phospho-Tyr216); GSK3B(phospho-Y216); p-GSK-3 beta(Tyr216); p-GSK-3 beta(Y216); Glycogen synthase kinase 3 beta; GSK 3 beta; GSK 3B; GSK3B; GSK3B protein; GSK3beta isoform; GSK3 beta; Glycogen synthase kinase-3 beta; GSK-3 beta; GSK3B_HUMAN.

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Phospho-GSK3 Beta (Tyr216) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (phospho-GSK3 Beta (Tyr216)) Polyclonal Antibody, Unconjugated (orb5359) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Mouse NIH/3T3 cell lysates, Lane 4: Rat Cerebrum tissue lysates, Lane 5: Rat Testis tissue lysates, Primary: Anti-phospho-GSK3 Beta (Tyr216) (orb5359) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kDa, Observed band size: 50 kDa.

Sample: Testis (Mouse) Lysate at 40 ug, Thymus (Mouse) Lysate at 40 ug, NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-phospho-GSK3 Beta (Tyr216) (orb5359) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 49 kD.

Quick Database Links

Gene Symbol

GSK3B

UniProt

P49841

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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