Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human IGF1R around the phosphorylation site of Tyr1165+Tyr1166 TD(p-Y)(p-Y)RK
Target	IGF1R
Molecular Weight	150 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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IGF1 Receptor(phospho Y1165+Y1166); p-IGF1 Receptor(phospho Y1165+Y1166); IGF-1R(phospho-Tyr1165/Tyr1166); IGF1R(phospho Y1165+Y1166); IGF1R(phospho Tyr1165+Tyr1166); p-IGF1R(Tyr1165+Tyr1166); phospho-IGF1R(Tyr1166+Tyr1167)rat; phospho-IGF1R(Tyr1166+Tyr1167)mo; Insulin-like growth factor 1 receptor; CD221; CD221 antigen; IGF1 Receptor; IGF 1 receptor; IGF 1R; IGF I receptor; IGFIRC; Insulin like growth factor 1 receptor precursor; JTK13; MGC142170; MGC142172; Insulin-like growth factor 1 receptor beta chain; IGF 1 receptor; IGF-1R; IGF I receptor; IGF-I receptor; IGF-IR; IGF1R_HUMAN.

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Phospho-IGF1R (Tyr1165 + Tyr1166) Rabbit Polyclonal Antibody

Blank control: Mouse Kidney (blue). Primary Antibody: Rabbit Anti-phospho-IGF1R (Tyr1165 + Tyr1166) antibody (orb5505, Green), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde for 10 min at 37°C. Primary antibody (orb5505, 1 µg/1x10^6 cells) were incubated for 30 min at room temperature, followed by 1X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min on ice. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

IGF1R

UniProt

P08069

UniProt Details

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No UniProt data available

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Protocol Information

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