Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human IL-1R1 around the phosphorylation site of Tyr496 QD(p-Y)EK
Target	IL1R1
Molecular Weight	63 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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IL1 Receptor I(phospho Tyr496); Antigen CD121a; CD121 antigen-like family member A; CD121A; CD121a antigen; D2S1473; IL 1; IL 1R 1; IL 1R alpha; IL 1RT1; IL-1R-1; IL-1R-alpha; IL-1RT-1; IL-1RT1; IL1 inhibitor; IL1R; IL1R1; IL1R1_HUMAN; IL1RA; IL1RN; IL1RT1; Interleukin 1 receptor alpha type 1; Interleukin 1 receptor antagonist protein(IRAP); Interleukin 1 receptor type I; Interleukin receptor 1; Interleukin-1 receptor alpha; Interleukin-1 receptor type 1; Interleukin-1 receptor type I; P80; IL1 Receptor I; IL-1 Receptor I; Interleukin-1 receptor type 1, soluble form; IL-1 sRI.

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Phospho-IL-1R1 (Tyr496) Rabbit Polyclonal Antibody

Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4°C). Primary Antibody (green line): Rabbit Anti-phospho-IL-1R1 (Tyr496) antibody (orb6228), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Sample: Intestine (Mouse) Lysate at 40 ug, Primary: Anti-phospho-IL-1R1 (Tyr496) (orb6228) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 63 kD, Observed band size: 63 kD.

Quick Database Links

Gene Symbol

IL1R1

UniProt

P14778

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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