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Phospho-ITGB2 (Thr758) Rabbit Polyclonal Antibody
SKU: orb156299
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IF, IHC-Fr, IHC-P |
|---|---|
| Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Equine, Gallus, Guinea pig, Mouse, Porcine, Rabbit, Rat, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthesised phosphopeptide derived from human CD18 around the phosphorylation site of Thr758 SA(p-T)TT |
| Target | ITGB2 |
| Molecular Weight | 82 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Integrin beta-2; integrin subunit beta 2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunit beta; Complement receptor C3 subunit beta;
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Blank control: Raji cells (blue). Primary Antibody: Rabbit Anti-Phospho-CD18 (Thr758) antibody (orb156299), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.
Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-CD18 (Thr758) Polyclonal Antibody, Unconjugated (orb156299) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-CD18 (Thr758) Polyclonal Antibody, Unconjugated (orb156299) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-CD18 (Thr758) Polyclonal Antibody, Unconjugated (orb156299) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Quick Database Links
Gene Symbol
ITGB2
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
Phospho-ITGB2 (Thr758) Rabbit Polyclonal Antibody (orb156299)
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