Phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221) Rabbit Polyclonal Antibody

SKU: orb312293

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Description

Research Area

Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Porcine

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human JNK1/JNK2/JNK3 around the phosphorylation site of T183/T183/T221 MM(p-T)PY
Target	MAPK8
Molecular Weight	42 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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JNK1 & JNK2 & JNK3 (p-T183/T183/T221); p-JNK1 & JNK2 & JNK3; phospho-JNK1 & JNK2 & JNK3; JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2; PRKM8; SAPK1; SAPK1c; MK08_HUMAN; MAPK8; MAP kinase 8; MAPK 8; Stress-activated protein kinase 1c (SAPK1c); Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; 2.7.11.24; MK08_MOUSE; MK08_RAT; SAPK gamma; p54 gamma;

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Phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221)) Polyclonal Antibody, Unconjugated (orb312293) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MAPK8) Polyclonal Antibody, Unconjugated (orb312293) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221)) Polyclonal Antibody, Unconjugated (orb312293) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Rat) Lysate at 40 ug, Lane 4: Large intestine (Mouse) Lysate at 40 ug, Lane 5: Uterus (Mouse) Lysate at 40 ug, Lane 6: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 7: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221) (orb312293) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46/54 kD, Observed band size: 46 kD.

Quick Database Links

Gene Symbol

MAPK8

UniProt

P45983

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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