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Description
Research Area
Signal Transduction
Images & Validation
−
Item 1 of 4
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Equine, Monkey, Porcine, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthesised phosphopeptide derived from human Parkin around the phosphorylation site of Ser65 QQ(p-S)IV |
| Target | PRKN |
| Molecular Weight | 50 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−AR-JP; LPRS2; PARK2; PDJ; PRKN_HUMAN; PRKN; Parkin; Parkin RBR E3 ubiquitin-protein ligase; Parkinson juvenile disease protein 2 (Parkinson disease protein 2); 2.3.2.31; parkin RBR E3 ubiquitin protein ligase; Parkinson disease (autosomal recessive, juvenile) 2, parkin; parkinson protein 2, E3 ubiquitin protein ligase (parkin); E3 ubiquitin ligase; PRKN | Parkin (phospho-S65); p-Parkin; phospho-Parkin; p-PRKN; PRKN | Parkin (phospho-Ser65)
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Quick Database Links
Gene Symbol
PRKN
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Filter by Applications
Filter by Species
Chakrabarti, Lina et al. Mitochondrial membrane potential-enriched CHO host: a novel and powerful tool for improving biomanufacturing capability MAbs, 14, 2020081 (2022)
Jamie Whitfield 1, Alannah K A McKay 2, Nicolin Tee 2, Rachel McCormick 2, Aimee Morabito 2, Leonidas G Karagounis 2 3 4, Andréane M Fouassier 5, Davide D'Amico 5, Anurag Singh 5, Louise M Burke 2, John A Hawley 2 6 Evaluating the Impact of Urolithin A Supplementation on Running Performance, Recovery, and Mitochondrial Biomarkers in Highly Trained Male Distance Runners Sports Medicine, (2025)
Applications
WB
Reactivity
Human
Huang, Yi-Fan et al. The protective effort of GPCR kinase 2-interacting protein-1 in neurons via promoting Beclin1-Parkin induced mitophagy at the early stage of spinal cord ischemia-reperfusion injury FASEB J, 34, 2055-2074 (2020)
Yu, Shao-Hua et al. Human antigen R regulates hypoxia-induced mitophagy in renal tubular cells through PARKIN/BNIP3L expressions J Cell Mol Med, 25, 2691-2702 (2021)
Lane, Sydney L. et al. Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in Mice Int J Mol Sci, 22, 13019 (2021)
Sun, Xin et al. Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy Acta Pharm Sin B, 12, 838-852 (2022)
Zhang, Qin et al. Regulation of mitophagy through HIF-1?/miR-140-5p/PARKIN axis in acute kidney injury Environ Toxicol, 37, 1759-1767 (2022)
Rai, Rajani et al. Distinct mechanism of cervical cancer cell death caused by the investigational new drug SHetA2 Front Oncol, 12, 958536 (2022)
Applications
IHC
Reactivity
Porcine
Singh, Anurag et al. Urolithin A improves muscle strength, exercise performance, and biomarkers of mitochondrial health in a randomized trial in middle-aged adults Cell Rep Med, 3, 100633 (2022)
Chao, Tony et al. Burn-Induced Reductions in Mitochondrial Abundance and Efficiency are More Pronounced with Small Volumes of Colloids in Swine Am. J. Physiol., Cell Physiol., (2019)
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