Phospho-PLCB3 (Ser1105) Rabbit Polyclonal Antibody

SKU: orb6737

This product can be custom made

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Description

Phospho-PLCB3 (Ser1105) Rabbit Polyclonal Antibody

Research Area

Metabolism Research

Images & Validation

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Item 1 of 2

Tested Applications	FC, ICC
Dilution Range	ICC/IF=1:100-500, Flow-Cyt=1μg /Test
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Mouse, Porcine, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthesised phosphopeptide derived from human PLC beta3 around the phosphorylation site of Ser1105 N(p-S)ISE
Target	PLCB3
Molecular Weight	136 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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PLCB3 (p-S1105); p-PLCB3; phospho-PLCB3; SMDCD; mKIAA4098; PLCB3_HUMAN; PLCB3; Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3 (PLC-beta-3); 3.1.4.11; PLCB3_MOUSE; PLCB3_RAT;

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Phospho-PLCB3 (Ser1105) Rabbit Polyclonal Antibody

Blank control (blue): RAJI (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Phospho-PLC beta3 (Ser1105) antibody (orb6737), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

MCF-7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-PLC beta3 (Ser1105)) polyclonal Antibody, Unconjugated (orb6737) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

PLCB3

UniProt

Q01970

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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ICC

Immunocytochemistry

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