Phospho-TIRAP (Tyr86) Rabbit Polyclonal Antibody

SKU: orb100399

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Description

Images & Validation

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Item 1 of 5

Tested Applications	FC, IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
Reactivity	Human, Rat
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Mouse, Porcine

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human TIRAP around the acetylation site of Tyr86 KD(p-Y)DV
Target	TIRAP
Molecular Weight	24 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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TIRAP(phospho Y86); Adapter protein wyatt; MAL antibody MyD88 adapter like protein; TIR domain containing adapter protein; Toll interleukin 1 receptor(TIR) domain containing adaptor protein; Toll interleukin 1 receptor domain containing adapter protein; Toll like receptor adaptor protein; Wyatt.

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Phospho-TIRAP (Tyr86) Rabbit Polyclonal Antibody

Blank control: U937. Primary Antibody (green line): Rabbit Anti-phospho-TIRAP (Tyr86) antibody (orb100399), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (phospho-TIRAP (Tyr86)) Polyclonal Antibody, Unconjugated (orb100399) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (phospho-TIRAP (Tyr86)) Polyclonal Antibody, Unconjugated (orb100399) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (phospho-TIRAP (Tyr86)) Polyclonal Antibody, Unconjugated (orb100399) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human HUVEC cell lysates, Lane 3: Human U251 cell lysates, Lane 4: Human SH-SY5Y cell lysates, Lane 5: Human K562 cell lysates, Primary: Anti-phospho-TIRAP (Tyr86) (orb100399) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kDa, Observed band size: 35 kDa.

Quick Database Links

Gene Symbol

TIRAP

UniProt

P58753

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence