Phospho-TrkA (Tyr674 + Tyr675) + TrkB (Tyr706 + Tyr707) Rabbit Polyclonal Antibody

SKU: orb7120

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Description

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human TrkA around the phosphorylation site of Tyr674/675 TD(p-Y)(p-Y)RV
Target	NTRK1
Molecular Weight	90 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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TrkA(phospho-Tyr674/675); TrkA(phospho Tyr674/675); TrkA(phospho-Y674/675); p-TrkA(Tyr674/675); TrkB(phospho-Tyr706/707); TrkB(phospho Tyr706/707); TrkB(phospho-Tyr706/707); p-TrkB(Tyr706/707); TrkB(phospho Y706+Y707); CIPA; CIPA; DKFZp781I14186; gp140trk; High affinity nerve growth factor receptor; High affinity nerve growth factor receptor precursor; High affinity nerve growth factor receptor precursor; MTC; MTC; neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase receptor type 1; Ntrk 1; Ntrk1; Ntrk1; NTRK1_HUMAN; Oncogene TRK; Oncogene TRK; p140 TrkA; p140-TrkA; TRK 1; Trk A; TRK; TRK; Trk-A; TRK1; TRK1 transforming tyrosine kinase protein; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor A; Tyrosine kinase receptor; Tyrosine Receptor Kinase A.

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Phospho-TrkA (Tyr674 + Tyr675) + TrkB (Tyr706 + Tyr707) Rabbit Polyclonal Antibody

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Heart (Rat) Lysate at 40 ug, Lane 3: Large intestine (Rat) Lysate at 40 ug, Lane 4: Stomach (Rat) Lysate at 40 ug, Lane 5: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 6: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-TrkA (Tyr674 + Tyr675) + TrkB (Tyr706 + Tyr707) (orb7120) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 140/110 kD, Observed band size: 120 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) Polyclonal Antibody, Unconjugated (orb7120) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

NTRK1

UniProt

P04629

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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