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Description
Research Area
Cell Biology
Images & Validation
−
Item 1 of 2
| Tested Applications | IF, IHC-Fr, IHC-P |
|---|---|
| Dilution Range | IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500 |
| Reactivity | Human |
| Predicted Reactivity | Equine, Mouse, Rabbit, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from mouse ASC (101-190/193aa) |
| Target | PYCARD |
| Molecular Weight | 22 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ASC; CARD5; TMS; TMS-1; TMS1; ASC_HUMAN; PYCARD; hASC; Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1; PYD and CARD domain containing; apoptosis-associated speck-like; apoptosis-associated speck-like protein containing a CARD
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Quick Database Links
Gene Symbol
PYCARD
Protocol Information
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
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Sebastian Vogel, Taruna Arora, Xunde Wang, Laurel Mendelsohn, James Nichols, Darlene Allen, Arun S Shet, Christian A Combs, Zenaide M N Quezado, Swee Lay Thein The platelet NLRP3 inflammasome is upregulated in sickle cell disease via HMGB1/TLR4 and Bruton tyrosine kinase Blood Adv ., 2, 20 (2018)
Applications
IF
Reactivity
Human
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