AVS Bio - human citrullinated fibrinogen antibody

SKU: MQR2.101

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Description

Antibody against fibrinogen. Fibrinogen is a protein produced by the liver which helps stop bleeding by helping blood clots to form. Fibrinogen gets deiminated (conversion from arginin to citrullin) by Peptidyl Arginine Deiminase (PAD) in inflamed joints in patients that develop rheumatoid arthritis. Citrulline, while being an amino acid, is not built into proteins during protein synthesis, as it is not coded for by DNA, yet several proteins are known to contain citrulline. Proteins that normally contain citrulline residues include myelin basic protein (MBP), filaggrin, and several histone proteins, while other proteins, like fibrin and vimentin can get deiminated during cell death and tissue inflammation. Patients with rheumatoid arthritis often (at least 80% of them) develop an immune response against proteins containing citrulline. Although the origin of this immune response is not known, detection of antibodies reactive with citrulline containing proteins or peptides is now becoming an important help in the diagnosis of rheumatoid arthritis.

Images & Validation

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Tested Applications	ELISA, IB, IHC, IP
Dilution Range	ELISA: 1:200 – 1000. Immunoblotting: 1:200 – 1000. Other applications: since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need. Unless the stability in the actual test system has been established, it is recommended to dilute the product immediately before use.
Reactivity	Human

Key Properties

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Clonality	Monoclonal
Isotype	Human IgG1, λ
Clone No.	1F11
Target	citrullinated fibrinogen
Purification	Protein A purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	lyophilized
Buffer/Preservatives	The antibody has been lyophilized in a 10 mM ammonium bicarbonate buffer.
Expiration Date	For shelf life and expiration details, please refer to the Certificate of Analysis (CoA).
Disclaimer	For research use only

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