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CD163 Mouse Monoclonal Antibody (EDHu-1)

SKU: orb432914

Description

The CD163 Mouse Monoclonal Antibody (EDHu-1) is a purified, unconjugated IgG1 targeting human CD163. It is validated for ELISA, FC, IA, IF, IH-Fr, IHC-P, and WB applications.

Research Area

Immunology & Inflammation

Images & Validation

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Tested ApplicationsELISA, FC, IA, IF, IH-Fr, IHC-P, WB
Dilution RangeFC 1/10-1/50
ReactivityHuman
Application Notes
FC: Use 10μl of the suggested working dilution to label 10^6 cells in 100μl.

Key Properties

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Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.EDHu-1
ImmunogenLeucocytes harvested from the pleural cavity of patients with idiopathic spontaneous pneumothorax
TargetCD163
PurificationAffinity chromatography on Protein
ConjugationUnconjugated

Customer Validated Data

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The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution

Latest Experiments

15 Results
Dilution
Sample
Summary
IF
Human
-
Human retinal organoid cystic compartments
This antibody was used as a...
IHC
Human
-
Human skin biopsy specimens
This antibody was used as a...
IHC
Human
1:100
Full-thickness human 3D skin models
This antibody used as a primary...
IF
Sheep
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Sheep ileal tissue
This antibody was used as a...
Immunoblotting
Porcine
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Lysates of PK-15 cells transfected with wild-type or mutant pCD163.
This antibody was used as a...

Storage & Handling

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StorageIt is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.09% sodium azide
Concentration1.0 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Similar Products

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  • CD163 Mouse Monoclonal Antibody (EDHu-1) [orb432912]

    ELISA,  FC,  IA,  IF,  IHC-Fr,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    0.2 mg
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

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No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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25 μg
$ 230.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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