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Featured
Description
Research Area
Cell Biology
Images & Validation
−
Item 1 of 5
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:200 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Peptide-KLH. Peptide sequence around phosphorylation site of serine 2448 (T-D-S(p)-Y-S) derived from Human mTOR |
| Target | mTOR |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Concentration | 1.0mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−FRAP; FRAP1; FRAP2; RAFT1;
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Quick Database Links
Gene Symbol
mTOR
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
Filter by Applications
Filter by Species
Du, Chunyang et al. Thioredoxin-interacting protein regulates lipid metabolism via Akt/mTOR pathway in diabetic kidney disease Int J Biochem Cell Biol, 79, (2016)
Applications
WB
Reactivity
Mouse
Du, Chunyang et al. Protease-activated receptor-2 promotes kidney tubular epithelial inflammation by inhibiting autophagy via the PI3K/Akt/mTOR signalling pathway Biochem J, 474, 2733-2747 (2017)
Applications
WB
Reactivity
Mouse
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