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RACK1 Rabbit Polyclonal Antibody

SKU: orb6794

Description

RACK1 Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

Tested ApplicationsICC, IF, IHC-Fr, IHC-P
Dilution RangeIHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
ReactivityHuman, Rat
Predicted ReactivityBovine, Canine, Gallus, Mouse, Rabbit, Sheep

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human RACK1 (221-317/317aa)
TargetRACK1
Molecular Weight35 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

C12-3; GNB2L1; c12.3; Gnb2-rs1; H12.3; HLC-7; PIG21; GB-like; p205; RACK1_CHICK; RACK1; Guanine nucleotide-binding protein subunit beta-like protein 12.3; Receptor for activated C kinase; Receptor of activated protein C kinase 1; Receptor of activated protein kinase C 1 (RACK1); RACK1_BOVIN; Guanine nucleotide-binding protein subunit beta-2-like 1; Receptor of activated protein kinase C 1; GBLP_MACFA; RACK1_HUMAN; Cell proliferation-inducing gene 21 protein; Human lung cancer oncogene 7 protein (HLC-7); RACK1_MOUSE; 12-3; RACK1_PIG; RACK1_RAT;

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

RACK1 Rabbit Polyclonal Antibody

4% Paraformaldehyde-fixed HepG2 (H) cell, Triton X-100 at r.t. for 20 min, Antibody incubation with (RACK1) polyclonal Antibody, unconjugated (orb6794) 1:50, 90 min at 37°C, followed by conjugated Goat Anti-Rabbit IgG antibody (green, orb868805) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

RACK1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RACK1) Polyclonal Antibody, Unconjugated (orb6794) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

RACK1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat uterus tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RACK1) Polyclonal Antibody, Unconjugated (orb6794) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

UniProt Details

No UniProt data available

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol

RACK1 Rabbit Polyclonal Antibody (orb6794)

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Available Sizes

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50 μl
¥ 3,250.00
100 μl
¥ 5,070.00
200 μl
¥ 6,110.00
DispatchUsually dispatched within 5-10 working days
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