Rad51 Rabbit Polyclonal Antibody (BF555)

SKU: orb1975102

Description

Rad51 Rabbit Polyclonal Antibody (BF555) is a BF555 conjugated antibody targeting RAD51. This antibody is suitable for FC, ICC, IF. It exhibits reactivity with Human, Mouse, Rat samples.

Research Area

DNA and RNA Researchh, Homelogous Recombination

Images & Validation

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Tested Applications	FC, ICC, IF
Dilution range	IF=1:100-500, ICC/IF=1:100-500, Flow-Cyt=1ug/test
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Equine, Porcine, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Rad51 (81-180/339aa)
Target	RAD51
Purification	Affinity purified by Protein A
Conjugation	BF555

Storage & Handling

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Storage	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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BRCA1/BRCA2 containing complex, subunit 5; BRCC 5; BRCC5; DNA repair protein RAD51 homolog 1; DNA repair protein rhp51; E coli RecA homolog; HGNC:9817; Homolog of E coli RecA; homolog of S cerevisiae RAD51; HRAD51; HsRad51; HsT16930; Rad 51; RAD51 homolog(RecA homolog, E. coli)(S. cerevisiae); RAD51 homolog; RAD51 homolog S. cerevisiae; RAD51 S cerevisiae homolog; RAD51A; RECA; RecA homolog E. coli; RecA like protein; RecA, E. coli, homolog of; recombination protein A.

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Rad51 Rabbit Polyclonal Antibody (BF555)

Tissue/Cell: FHC cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal rabbit serum) at 37°C for 20 min, Incubation: Anti-Rad51 Polyclonal Antibody, conjugated (orb1975102) 1:50, 2 hours at 37°C, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: H9C2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal rabbit serum) at 37°C for 20 min, Incubation: Anti-Rad51 Polyclonal Antibody, conjugated (orb1975102) 1:50, 2 hours at 37°C, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

RAD51

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Protocol Information

Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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