RANKL/CD254 Rabbit Polyclonal Antibody

SKU: orb11190

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Description

Images & Validation

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Item 1 of 5

Tested Applications	FC, IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
Reactivity	Human, Mouse
Predicted Reactivity	Canine, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human OPGL (210-317/317aa)
Target	TNFSF11
Molecular Weight	35 kDa
Purification	Affinity purified by Protein A

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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OPGL; CD254; hRANKL2; ODF; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; RANKL; Receptor activator of nuclear factor kappa B ligand; sOdf; SOFA; TNF related activation induced cytokine; TNFSF 11; TNFSF11; TRANCE; Tumor necrosis factor ligand superfamily member 11; Osteoprotegerin Ligand; TNF11_HUMAN.

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Blank control: mouse splenocytes (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µl in 100 µl 1X PBS containing 0.5% BSA (green).

Sample: HUVEC-C Cell (Human) Lysate at 40 ug, HepG2 Cell (Human) Lysate at 40 ug, Primary: Anti-RANKL/CD254 (orb11190) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35kD, Observed band size: 56kD.

Sample: Lymph node (Mouse) Lysate at 40 ug, Thymus (Mouse) Lysate at 40 ug, Primary: Anti-RANKL'CD254 (orb11190) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37'50 kD, Observed band size: 50 kD.

Tissue/Cell: human gastric carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-OPGL/RANKL/ODF Polyclonal Antibody, Unconjugated (orb11190) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-OPGL/RANKL/ODF Polyclonal Antibody, Unconjugated (orb11190) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

TNFSF11

UniProt

O14788

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence

Filter by Applications

IHC (4)
WB (2)

Filter by Species

Rat (4)

Li, Jingwu et al. Effect of gallium nitrate on the expression of osteoprotegerin and receptor activator of nuclear factor‑κB ligand in osteoblasts in vivo and in vitro Mol Med Rep, 13, 769-777 (2016)

PubMed: 26647856

Applications

IHC, WB

Reactivity

Rat

HH Mustafa, SMA Hassan, SA Muhammad Influence of platelet-rich plasma on RANKL and IL-1 immunohistochemical expression in periodontitis-related bone cell proliferation and differentiation The Saudi Dental Journal, (2024)

PubMed:

Applications

IHC

Reactivity

Rat

Yuxiang Zhan 1, Siqin Zhang 2, Minjie Yang 3, Yuchen Wang 3, Jiaquan Li 4, Xiaolin Nong Artesunate Promotes Bone Remodeling Through TRAF6-Mediated NF-κB Signaling Under Orthodontic Stress in Diabetic Rats Int Dent J, (2025)

PubMed: 40393315

Applications

IHC

Reactivity

Rat

Jiao, Mengyu et al. Osteoprotegerin/receptor activator of nuclear factor-?B ligand are involved in periodontitis-promoted vascular calcification Exp Ther Med, 24, 512 (2022)

PubMed: 35813311

Applications

IHC, WB

Reactivity

Rat