RANKL/CD254 Rabbit Polyclonal Antibody

SKU: orb500861

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Description

Images & Validation

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Tested Applications	FC, WB
Dilution range	WB=1:500-2000, Flow-Cyt=1ug/Test
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Porcine, Rabbit, Rat, Zebrafish

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human RANKL/CD254 (41-140/317aa)
Target	TNFSF11
Molecular Weight	35 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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OPGL; CD254; hRANKL2; ODF; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; RANKL; Receptor activator of nuclear factor kappa B ligand; sOdf; SOFA; TNF related activation induced cytokine; TNFSF 11; TNFSF11; TRANCE; Tumor necrosis factor ligand superfamily member 11; Osteoprotegerin Ligand; TNF11_HUMAN.

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Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-RANKL/CD254 antibody (orb500861), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-CD254 (orb500861) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35 kD, Observed band size: 37/50 kD.

Sample: Lymph node (Mouse) Lysate at 40 ug, Thymus (Mouse) Lysate at 40 ug, Lymph node (Rat) Lysate at 40 ug, Primary: Anti-RANKL'CD254 (orb500861) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37'50 kD, Observed band size: 50 kD.

Quick Database Links

Gene Symbol

TNFSF11

UniProt

O14788

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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