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Product Reviews

Browse validated feedback from researchers employing Biorbyt products.

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Showing 219 reviews

Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use Western Blots and subsequent sequencing to screen potential knockout clones. Not bad for screening knockouts, but there is a nonspecific band close to the expected band which complicates analysis. I think the lower band is the correct band, though I can't be sure.

Tamanna Hossin's review image 1 for orb156964
Reactivity:Mouse
Application:WB
Tamanna HossinYale, Tech
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It is a good cell-surface blocking antibody(HTF1). I used it in one-stage and two-stage clotting assays with the conc. of 10 ug/ml in Human endothelial cells.

Saravanan Subramaniam's review image 1 for orb402784
Reactivity:Human
Application:Activity Assay
Saravanan SubramaniamVersiti-Blood Research Institute, Research Scientist
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We have used this CD133 antibody for quite some time, as it gives us highly reproducible results and easily identified staining. The concentration ratio allows us to do multiple sets of slides with the same lot and produces publication-quality results every time." Review date: 04 Sep 2020

Reactivity:Mouse
Application:IHC
Catherine Langford
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We performed CHIP-SICAP, a modified CHIP protocol that involves stringent wash to remove non-specific chromatin-associated proteins. So, labeling DNA with biotin followed by steptavidin is key to achieving high sensitivity. We evaluated the results by qPCR. We got great results with as little as four times of the cells as prescribed by other CHIP SICAP users.

Reactivity:Other
Application:ChIP
Charlesantony Aruljothi
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The product seems to be reliable and confers robust results, with reproductivity. The pricing is fair, although import fee and shipping times can be a nuisance. Good product overall.

Reactivity:Human
Application:WB
Ricardo SoaresUniversidade de São Paul
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The IFNAR1 antibody (orb5499) was effective and easy to titrate for accurate results in human FC test.

Reactivity:Human
Application:FC
CustomerUniversity of Miami
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"Great quality product. Antibody very high quality, achieved very high publication quality gels, no specificity issues and the protocol was spot on! Worked first time no optimization required."

Reactivity:Mouse
Application:WB
Alison DicksonSwansea University
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The chemical easily labeled nascent peptides even at lower concentrations. I have repeated the experiments three times, and the results are consistent.

Reactivity:Other
Application:Conjugation
Jie Tian
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