Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human RPS6KA1 (621-732/732aa)
Target	RPS6KA1
Molecular Weight	90 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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KS6A1_MOUSE; Rps6ka1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1 (p90-RSK 1 | p90RSK1 | p90S6K); MAP kinase-activated protein kinase 1a (MAPK-activated protein kinase 1a | MAPKAP kinase 1a | MAPKAPK-1a); Ribosomal S6 kinase 1 (RSK-1); 2.7.11.1; Mapkapk1a; Rsk1; KS6A1_RAT; HU-1; MAPKAPK1; RSK; p90Rsk;

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Tissue/Cell: A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (RPS6KA1) Polyclonal Antibody, Unconjugated (orb1499429) 1:200, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) at 37°C for 90 minutes, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Tissue/Cell: HeLa cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (RPS6KA1) Polyclonal Antibody, Unconjugated (orb1499429) 1:200, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) at 37°C for 90 minutes, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

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Gene Symbol

RPS6KA1

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Protocol Information

ICC

Immunocytochemistry

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