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SCNN1B Rabbit Polyclonal Antibody
SKU: orb4657
Featured
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ICC, WB |
|---|---|
| Dilution range | WB=1:500-2000, ICC/IF=1:100-500 |
| Reactivity | Human |
| Predicted Reactivity | Equine, Mouse, Porcine, Rabbit, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human SCNN1B (401-500/685aa) |
| Target | SCNN1B |
| Molecular Weight | 95-100/65-75 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Amiloride sensitive sodium channel subunit beta; beta NaCH; ENaC beta; ENaCB; Epithelial Na(+) channel subunit beta; Epithelial Na+channel beta subunit; Epithelial Na+channel subunit beta; Epithelial sodium channel beta 2 subunit; Epithelial sodium channel beta 3 subunit; Nonvoltage gated sodium channel 1 beta subunit; SCNNB_HUMAN; Nonvoltage gated sodium channel 1 subunit beta; SCNEB; SCNN 1B; Sodium channel nonvoltage gated 1 beta(Liddle syndrome); Sodium channel nonvoltage gated 1 beta.
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Sample: Lane 1: Human A673 cell lysates, Primary: Anti-SCNN1B (orb4657) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 73 kDa, Observed band size: 110 kDa.
U266 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (SCNN1B) polyclonal Antibody, Unconjugated (orb4657) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Quick Database Links
Gene Symbol
SCNN1B
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
SCNN1B Rabbit Polyclonal Antibody (orb4657)
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