SDHD Rabbit Polyclonal Antibody

SKU: orb6907

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Description

Research Area

Cell Biology, Energy Metabolism, Metabolism, Mitochondrial, Mitochondrial Markers, Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, WB
Dilution Range	WB=1:500-2000, ELISA=1:5000-10000
Reactivity	Mouse, Rat
Predicted Reactivity	Equine, Gallus, Human

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human SDHD (81-159/159aa)
Target	SDHD
Molecular Weight	11 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CBT1; CII 4; CII-4; CII4; CybS; DHSD_HUMAN; mitochondrial; OTTHUMP00000234720; OTTHUMP00000234721; OTTHUMP00000234722; OTTHUMP00000234723; OTTHUMP00000234724; OTTHUMP00000234725; OTTHUMP00000234726; PGL; PGL1; QPs3; SDH4; sdhD; Succinate dehydrogenase [ubiquinone] cytochrome b small subunit; Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial; Succinate dehydrogenase complex subunit D; Succinate dehydrogenase complex, subunit D, integral membrane protein; Succinate dehydrogenase ubiquinone cytochrome B small subunit; Succinate ubiquinone oxidoreductase cytochrome b small subunit; Succinate ubiquinone reductase membrane anchor subunit; Succinate-ubiquinone oxidoreductase cytochrome b small subunit; Succinate-ubiquinone reductase membrane anchor subunit.

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Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Mouse Heart tissue lysates, Lane 3: Rat Kidney tissue lysates, Primary: Anti-SDHD at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 11 kDa, Observed band size: 11 kDa.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-SDHD Polyclonal Antibody, Unconjugated (orb6907) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

SDHD

UniProt

O14521

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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WB (1)

Filter by Species

Rat (1)

Mo, Yulin et al. TXNIP contributes to bone loss via promoting the mitochondrial oxidative phosphorylation during glucocorticoid-induced osteoporosis Life Sci, 266, 118938 (2021)

PubMed: 33347878

Applications

Reactivity

Rat