SOCS3 Rabbit Polyclonal Antibody

SKU: orb499629

This product can be custom made

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Description

SOCS3 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Cytokine Research, Cytoplasmic, Hypoxia, Interleukin-6 (IL-6) related, JAK/ STAT Signaling Pathway, Obesity

Images & Validation

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Item 1 of 2

Tested Applications	FC, IF, IHC-Fr, IHC-P
Dilution Range	IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
Reactivity	Human, Rat
Predicted Reactivity	Bovine, Canine, Equine, Gallus, Mouse, Porcine

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human SOCS3 1/80/225
Target	SOCS3
Molecular Weight	25 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ATOD4; CIS3; Cish3; SOCS-3; SSI-3; SSI3; SOCS3_HUMAN; SOCS3; Cytokine-inducible SH2 protein 3 (CIS-3); STAT-induced STAT inhibitor 3 (SSI-3); SOCS3_RAT; Cytokine-inducible SH2 protein 3;

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Blank control (blue line): Hep G2 (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% methanol for 20 min at -20°C). Primary Antibody (green line): Rabbit Anti-SOCS3 antibody (orb499629), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SOCS3) Polyclonal Antibody, Unconjugated (orb499629) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

SOCS3

UniProt

O14543

UniProt Details

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No UniProt data available

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Datasheet

Product Information

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

View Protocol

IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence