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SPECA Rabbit Polyclonal Antibody (Cy5.5)

SKU: orb1049214

Description

SPECA Rabbit Polyclonal Antibody (Cy5.5) is a Cy5.5 conjugated antibody targeting SPTAN1. This antibody is suitable for IF.

Research Area

Actin Crosslinking, Cell Biology, Neurology, Neuroscience

Images & Validation

Tested ApplicationsIF
Dilution rangeIF=1:100-500
Predicted ReactivityBovine, Canine, Equine, Gallus, Human, Mouse, Porcine, Rabbit, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human SPECA (551-650/2472aa)
TargetSPTAN1
PurificationAffinity purified by Protein A
ConjugationCy5.5

Storage & Handling

StorageShipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

Fodrin alpha chain; (ALPHA)II-SPECTRIN; alpha II Spectrin; FLJ44613; Fodrin, alpha; NEAS; Non erythrocytic spectrin alpha; SPECA; Spectrin alpha chain; Spectrin alpha chain brain; Spectrin alpha non erythrocytic 1; Spectrin non erythroid alpha chain; Spectrin, alpha, non-erythrocytic 1(alpha-fodrin); Spectrin, nonerythroid, alpha subunit; Spna2; SPTA 2; SPTA2; SPTAN 1; SPTAN1; SPTA1_HUMAN.

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SPECA Rabbit Polyclonal Antibody (Cy5.5)

Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-SPECA antibody (orb1049214), Dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IGg-Cy5.5. Dilution: 3 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

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Protocol Information

IF
Immunofluorescence
View Protocol

SPECA Rabbit Polyclonal Antibody (Cy5.5) (orb1049214)

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Available Sizes

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100 μl
¥ 6,500.00
DispatchUsually dispatched within 2 to 3 weeks.
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