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Description
Research Area
Images & Validation
−| Tested Applications | IF, IHC-Fr, IHC-P |
|---|---|
| Dilution Range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Mouse |
| Predicted Reactivity | Bovine, Human, Rabbit, Rat, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Stabilin 2 (2201-2300/2551aa) |
| Target | STAB2 |
| Molecular Weight | 154/275 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Tissue/Cell: mouse spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Stabilin 2 Polyclonal Antibody, Unconjugated (orb158499) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.
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UniProt Details
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Protocol Information
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Fajardo, Val A. et al. Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice PLoS One, 12, e0173708 (2017)
Applications
Reactivity
Fajardo, Val A. et al. Effects of sarcolipin deletion on skeletal muscle adaptive responses to functional overload and unload Am J Physiol Cell Physiol, 313, C154-C161 (2017)
Applications
Reactivity
Fajardo, Val A. et al. Sarcolipin deletion in mdx mice impairs calcineurin signalling and worsens dystrophic pathology Hum Mol Genet, 27, 4094-4102 (2018)
Applications
Reactivity
Stabilin 2 Rabbit Polyclonal Antibody (orb158499)
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