Thrap3 Rabbit Polyclonal Antibody

SKU: orb1855

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Description

Thrap3 Rabbit Polyclonal Antibody

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200
Reactivity	Human, Rat
Predicted Reactivity	Bovine, Canine, Equine, Mouse, Porcine, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Thrap3/TRAP150 (631-730/955aa)
Target	THRAP3
Molecular Weight	108 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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BCLAF2; TRAP150; 9330151F09Rik; B230333E16Rik; TR150_HUMAN; THRAP3; BCLAF1 and THRAP3 family member 2; Thyroid hormone receptor-associated protein complex 150 kDa component (Trap150); TR150_MOUSE; TR150_XENLA;

Quality Guarantee

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Sample: Hela (Human) Cell Lysate at 30 ug, MOLT-4 (Human) Cell Lysate at 30 ug, Siha (Human) Cell Lysate at 30 ug, Primary: Anti-Thrap3 (orb1855) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 108 kD, Observed band size: 108 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Thrap3/TRAP150 Polyclonal Antibody, Unconjugated (orb1855) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

THRAP3

UniProt

Q9Y2W1

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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