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TP53 Rabbit Polyclonal Antibody
SKU: orb573620
Description
Research Area
Cancer Biology, Cell Biology, Epigenetics & Chromatin, Molecular Biology
Images & Validation
−Item 1 of 3
| Tested Applications | ChIP, ELISA, WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human TP53 |
| Target | TP53 |
| Molecular Weight | 44kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Concentration | 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−P53, BCC7, LFS1, BMFS5, TRP53
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Sample Type:human cystic fibrosis bronchial epithelial cells, P53: 1/1000Goat anti-rabbit-HRP: 1:5000.
U20S (p53+) cells were treated with 0.5 uM Doxorubicin for 14 hrs to induce DNA damage and hence activate p53. In parallel, PLKO cells (U2OS cells with stable shRNA-mediated knockdown of p53) were treated similarly and were used as negative control. Thedata for p21 promoter were normalised to actin (control for non-specific binding of DNA to the antibodies).
WB Suggested Anti-TP53 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:62500, Positive Control: 293T cell lysate, TP53 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ChIP
Chromatin Immunoprecipitation
TP53 Rabbit Polyclonal Antibody (orb573620)
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