TTF1/NKX2-1 Rabbit Polyclonal Antibody

SKU: orb11533

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Description

Images & Validation

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Item 1 of 2

Tested Applications	FC, ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, Flow-Cyt=1μg/Test, IHC-F=1:100-500, IF=1:100-500, ICC/IF=1:50-200
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human TTF-1 (201-300/372aa)
Target	NKX2-1
Molecular Weight	38 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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BCH; BHC; NK-2; NKX2.1; NKX2A; NMTC1; T/EBP; TEBP; TITF1; TTF-1; TTF1; NKX21_HUMAN; NKX2-1; Homeobox protein NK-2 homolog A; Thyroid nuclear factor 1; Thyroid transcription factor 1 (TTF-1); Thyroid-specific enhancer-binding protein (T/EBP); NKX21_MOUSE; Nkx-2.1; NKX21_RAT;

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Sample: Lane 1: Mouse Thyroid gland Lysates, Lane 2: Rat Thyroid gland Lysates, Primary: Anti-TTF1 (orb11533) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 38kDa, Observed band size: 38kDa.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TTF1/NKX2.1 Polyclonal Antibody, Unconjugated (orb11533) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

NKX2-1

UniProt

P43699

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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