Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human TTF1 (121-220/371aa)
Target	NKX2-1
Molecular Weight	38 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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NKX21_HUMAN; Homeobox protein Nkx-2.1; Homeobox protein NK-2 homolog A; Thyroid nuclear factor 1; Thyroid transcription factor 1(TTF-1); Thyroid-specific enhancer-binding protein(T/EBP); NKX2A; TITF1; TTF1; TTF-1; NK2 homeobox 1; BCH; BHC; NK-2; TEBP; NMTC1; T/EBP;

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Blank control (blue): RSC96 Cells (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-TTF1/AF647 Conjugated antibody, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions.

Cell: A549, Concentration: 1:100, Host/Isotype: Rabbit/IgG (blue), Flow cytometric analysis of Rabbit IgG isotype control (Cat#: orb186420) on A549 (green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody.

Sample: Lane 1: Mouse Thyroid gland tissue lysates, Primary: Anti-TTF1 (orb186420) at 1/200 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 38 kDa, Observed band size: 42 kDa.

Quick Database Links

Gene Symbol

NKX2-1

UniProt

P43699

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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