VDAC3 Rabbit Polyclonal Antibody

SKU: orb101384

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Description

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Mouse, Rat
Predicted Reactivity	Bovine, Canine, Equine, Human, Porcine, Rabbit, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human VDAC3 (101-200/283aa)
Target	VDAC3
Molecular Weight	31 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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hVDAC3; mVDAC3; Outer mitochondrial membrane protein porin 3; voltage dependent anion channel 3; VDAC3_HUMAN.

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Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Mouse Spleen tissue lysates, Lane 3: Rat Kidney tissue lysates, Lane 4: Rat Spleen tissue lysates, Primary: Anti-VDAC3 (orb101384) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kDa, Observed band size: 31 kDa.

Sample: Large intestine (Rat) Lysate at 40 ug, Primary: Anti-VDAC3 (orb101384) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.

Tissue/cell: rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-VDAC3 Polyclonal Antibody, Unconjugated (orb101384) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

VDAC3

UniProt

Q9Y277

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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Filter by Applications

CoIP (1)
IF (1)

Filter by Species

Mouse (1)

Wei Meng 1, Linghua Li N6-methyladenosine modification of SPOP relieves ferroptosis and diabetic cardiomyopathy by enhancing ubiquitination of VDAC3 Free Radic Biol Med, (2024)

PubMed: 39549880

Applications

CoIP, IF

Reactivity

Mouse