VE Cadherin Rabbit Polyclonal Antibody

SKU: orb158708

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Description

Research Area

Cadherins, Cardiovascular, Cardiovascular Research, Cell Biology, Cell Markers, Cell adhesion Molecules, Endothelial, Endothelial Cells, Varscular, Varscular System, Vascular

Images & Validation

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Tested Applications	FC
Dilution Range	Flow-Cyt=1μg/Test
Reactivity	Mouse
Predicted Reactivity	Bovine, Equine, Human, Porcine, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human VE Cadherin (251-320/784aa)
Target	CDH5
Molecular Weight	81 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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VE-cadherin; 7B4 antigen; VECD; Vascular endothelial cell cadherin; 7B 4; 7B4; 7B4 antigen; Cadherin 5; Cadherin 5 type 2; Cadherin-5; Cadherin5; CD 144; CD144; CD144 antigen; CDH 5; CDH5; CDH5 protein; Vascular endothelial cadherin; VE Cad antibody VEC; CADH5_HUMAN.

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Blank control (blue line): Mouse kidney (blue). Primary Antibody (green line): Rabbit Anti-VE Cadherin antibody (orb158708), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): F (ab')2 fragment goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 70% ice-cold methanol overnight at 4°C. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

CDH5

UniProt

P33151

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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