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VIM Rabbit Polyclonal Antibody
SKU: orb580337
Description
Research Area
Epigenetics, Infectious Diseases
Images & Validation
−Item 1 of 5
| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Sheep |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human VIM |
| Target | VIM |
| Molecular Weight | 54kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Concentration | 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−FLJ36605
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Sample Tissue: Mouse Small Intestine, Antibody dilution: 1 ug/ml.
Rabbit Anti-VIM Antibody, Catalog Number: orb580337, Formalin Fixed Paraffin Embedded Tissue: Human Ovary Tissue, Observed Staining: Cytoplasm, Primary Antibody Concentration: 1:100, Other Working Concentrations: N/A, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.
Sample Type: Human brain stem cells, Primary Antibody dilution: 1:500, Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594, Secondary Antibody dilution: 1:1000, Color/Signal Descriptions: VIM: Red DAPI:Blue, Gene Name: VIM.
The Vimentin antibody orb580337 works well for visualization of the vimentin cytoskeleton. We used PFA-fixed COS-7 cells and an antibody dilution of 1:400.
WB Suggested Anti-VIM Antibody Titration: 0.2-1 ug/ml, Positive Control: Human brain.
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
VIM Rabbit Polyclonal Antibody (orb580337)
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