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Description
Research Area
Musculoskeletal & Connective Tissue Research
Images & Validation
−
| Tested Applications | ELISA, FC, IP, WB |
|---|---|
| Dilution Range | Western blot, 0.25-0.5 μg/ml, Human Immunoprecipitation, 0.5-2 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml |
| Reactivity | Human |
| Application Notes |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human MIB2 recombinant protein (Position: A71-I572). Human MIB2shares 90.3% and 89.7% amino acid (aa) sequence identity with mouse and rat MIB2, respectively. |
| Target | E3 ubiquitin-protein ligase MIB2 |
| Molecular Weight | 110 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−MIB2; SKD; ZZANK1; E3 ubiquitin-protein ligase MIB2; EC 2.3.2.27; Mind bomb homolog 2; Novel zinc finger protein; Novelzin; Putative NF-kappa-B-activating protein 002N; RING-type E3 ubiquitin transferase MIB2; Skeletrophin; Zinc finger ZZ type with ankyrin repeat domain protein 1
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Quick Database Links
Gene Symbol
E3 ubiquitin-protein ligase MIB2
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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