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MIB2 Rabbit Polyclonal Antibody (Carrier-free)

SKU: orb3100733

Description

Anti-MIB2 Antibody. Tested in WB, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

Tested ApplicationsELISA, FC, IP, WB
Dilution RangeWestern blot, 0.25-0.5 μg/ml, Human Immunoprecipitation, 0.5-2 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml
ReactivityHuman
Application Notes
For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenE.coli-derived human MIB2 recombinant protein (Position: A71-I572). Human MIB2shares 90.3% and 89.7% amino acid (aa) sequence identity with mouse and rat MIB2, respectively.
TargetE3 ubiquitin-protein ligase MIB2
Molecular Weight110 kDa
PurificationImmunogen affinity purified.
ConjugationCarrier-free

Storage & Handling

StorageAt -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

MIB2; SKD; ZZANK1; E3 ubiquitin-protein ligase MIB2; EC 2.3.2.27; Mind bomb homolog 2; Novel zinc finger protein; Novelzin; Putative NF-kappa-B-activating protein 002N; RING-type E3 ubiquitin transferase MIB2; Skeletrophin; Zinc finger ZZ type with ankyrin repeat domain protein 1
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
View Protocol

Available Sizes

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100 μg
$ 470.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2-4 weeks
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