Recombinant Bcl2 Antibody

SKU: orb2640496

Description

Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies.

Images & Validation

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Tested Applications	FACS, IF, IHC-P, WB
Dilution range	Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the recombinant Bcl2 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rBCL2/782
Immunogen	Recombinant full-length human protein was used as the immunogen for the recombinant Bcl2 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using recombinant Bcl2 antibody (clone rBCL2/782). These results demonstrate the foremost specificity of the rBCL2/782 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of human follicular lymphoma with recombinant Bcl2 antibody (rBCL2/782). HIER: boil tissue sections in 1mM EDTA, pH8, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant Bcl2 antibody (clone rBCL2/782) as confirmation of integrity and purity.

Western blot testing of human MCF7 cell lysate with recombinant Bcl2 antibody (clone rBCL2/782). Expected molecular weight ~26 kDa.

IHC testing of human tonsil tissue with recombinant Bcl2 antibody (rBCL2/782). HIER: boil tissue sections in 1mM EDTA, pH8, for 10-20 min followed by cooling at RT for 20 min.

Quick Database Links

UniProt

P10415

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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