E-cadherin rabbit pAb

SKU: orb765095

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Description

Rabbit polyclonal antibody to E-cadherin

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IF, IHC-P, WB
Dilution range	Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human Cadherin. AA range:833-882
Molecular Weight	125-130
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-CDH1 antibody, anti-CDHE antibody, anti-UVO antibody, anti-Cadherin-1 antibody, anti-CAM 120/80 antibody, anti-Epithelial cadherin antibody, anti-E-cadherin antibody, anti-Uvomorulin antibody, anti-CD antigen CD324 antibody, anti-CDH2 antibody, anti-CDHN antibody, anti-NCAD antibody, anti-Cadherin-2 antibody, anti-CDw325 antibody, anti-Neural cadherin antibody, anti-N-cadherin antibody, anti-CD antigen CD325 antibody, anti-CDH3 antibody, anti-CDHP antibody, anti- antibody

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Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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