EpCAM / CD326 (Epithelial Marker) Antibody

SKU: orb750871

Description

Mouse monoclonal antibody against EpCAM conjugated to Biotin

Images & Validation

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Item 1 of 2

Tested Applications	FC, IF, IHC
Dilution range	Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood); Immunofluorescence (2-4ug/ml);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT);,(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Reactivity	Human
Application Notes	Positive Control: HT29 cells or breast tumor

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	VU-1D9
Immunogen	Small cell lung carcinoma cells
Target	EPCAM
Source/Expression System	Bioreactor
Molecular Weight	40-43kDa
Purification	Protein G
Conjugation	Biotin

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Buffer/Preservatives	Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Concentration	Purified Ab conjugated to Biotin
Disclaimer	For research use only

Alternative Names

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Anti-Adenocarcinoma-associated Antigen antibody, anti-Cell Surface Glycoprotein Trop-1 antibody, anti-EGP2 antibody, anti-EGP314 antibody, anti-EGP40 antibody, anti-Epithelial Cell Adhesion Molecule antibody, anti-Epithelial Glycoprotein 314 antibody, anti-ESA antibody, anti-KSA antibody, anti-TACD1 antibody, anti-TROP1 antibody, anti-Tumor-associated Calcium Signal Transducer 1 (TACSTD1) antibody, anti-ECS-1 antibody, anti-Epidermal Surface Antigen 1 antibody, anti-ESA1 antibody, anti-FLOT2 antibody, anti-Flotillin-2 antibody, anti-Membrane Component antibody, anti-Chromosome 17 antibody, anti-Surface Marker-1 (M17S1) antibody, anti-REG-1 antibody, anti-Reggie-1 antibody, anti-Reggie-2 antibody

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EpCAM / CD326 (Epithelial Marker) Antibody

Flow Cytometry of HT29 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Ep-CAM Monoclonal Antibody (SPM134).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Biotin-conjugated Ep-CAM Mouse Monoclonal Antibody (VU-1D9).

Quick Database Links

Gene Symbol

EPCAM

UniProt

P16422

NCBI Gene ID

4072

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

IHC

Immunohistochemistry

Flow Cytometry

Immunofluorescence