Fab Human IgG (H&L) Antibody

Fluorescence microscopy using Rabbit Fab Anti-Human IgG. Analysis of human IgGs displayed on the surface of OCMS™ hybridomas. (a) 8C5 OCMS™ (top row) and B5-6T OCMS™ (bottom row) cells were incubated overnight with the RAH (AB14), followed by biotinylated rabies GP and Alexa Fluor 488® streptavidin (green) (AB3), and visualized by fluorescence microscopy. Images are shown with DAPI (blue) (right) or without DAPI (left). Scale bar = 5 µm. (b) The 4G4 OCMS™ (top row) and 8C5 OCMS™ (bottom row) hybridomas were incubated overnight with a rabbit Fab anti-human IgG (AB17), then for an hour with additional rabbit Fab anti-human IgG and Sabin type III PV (left column) or GP (right column), followed by an Alexa Fluor® 488 anti-human IgG (green) (AB2). Nuclei were stained with DAPI (blue). Scale bar = 10 µm. (c) In this mixing experiment, non-secretor OCMS™ cell lines were labeled with CFSE (green) and mixed in a 10:1 ratio with IgG-secreting OCMS™ hybridomas. Cells were incubated overnight with rabbit F(ab')2 anti-human IgG (AB15) then washed and incubated with biotinylated Protein A (AB12) and APC streptavidin (red) (AB4). (Top row) 8C5 OCMS™ vs. B5-6T OCMS™ CFSE. (Bottom row) 4G4 OCMS™ vs. LCX OCMS™ CFSE. With DAPI (blue) (right column) or without (left column). Scale bar = 5 µm.