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GIT2 rabbit pAb
SKU: orb765302
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Description
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−Item 1 of 4
| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GIT2. AA range:361-410 |
| Molecular Weight | 84 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-GIT2 antibody, anti-KIAA0148 antibody, anti-ARF GTPase-activating protein GIT2 antibody, anti-ARF GAP GIT2 antibody, anti-Cool-interacting tyrosine-phosphorylated protein 2 antibody, anti-CAT-2 antibody, anti-CAT2 antibody, anti-G protein-coupled receptor kinase-interactor 2 antibody, anti-GRK-interacting prote antibody
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Western Blot analysis of various cells using GIT2 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.
Western Blot analysis of HepG2 cells using GIT2 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.
Immunohistochemical analysis of paraffin-embedded Human cervix cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blot analysis of lysates from HepG2 cells, using GIT2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
GIT2 rabbit pAb (orb765302)
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