Goat anti-Silver homologue / Pmel 17 (C-terminus) Antibody

SKU: orb19282

Description

Goat polyclonal antibody to SILV

Images & Validation

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Item 1 of 2

Tested Applications	IHC
Dilution range	ELISA: 1:16000, WB: 1-3μg/ml, IF/ICC: 10μg/ml
Reactivity	Human, Mouse
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 70-75kDa band observed in Human Skin lysates (calculated MW of 70.3kDa according to NP_008859.1). Recommended concentration: 1-3 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Silver homologue / Pmel 17 (C-terminus)
Molecular Weight	70.3
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti silver homolog (mouse) antibody, anti PMEL17 antibody, anti SILV antibody, anti D12S53E antibody, anti ME20 antibody, anti SI antibody, anti SIL antibody, anti gp100 antibody, anti Melanocyte protein mel 17 antibody, anti Pmel 17 antibody, anti Silver, mouse, homolog of antibody, anti silver (mouse homolog) like antibody, anti silver (mouse homolog)-like antibody, anti silver homolog antibody

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Goat anti-Silver homologue / Pmel 17 (C-terminus) Antibody

7 μg/ml staining of paraffin embedded Human Melanoma. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Melanoma, with no primary antibody.

Quick Database Links

Gene Symbol

Silver homologue / Pmel 17 (C-terminus)

NCBI Gene ID

6490

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

IHC

Immunohistochemistry

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