Goat anti-TBK1 (aa514-527) Antibody

SKU: orb137099

Description

Goat polyclonal to TBK1

Images & Validation

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Tested Applications	ELISA, FC, IF, WB
Dilution range	ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
Reactivity	Human
Application Notes	Approx 85kDa band observed in lysates of cell line HeLa (calculated MW of 83.6kDa according to NP_037386.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 24kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field-have any such results been reported with other antibodies/lysates. Have any further splice variants/modified forms been reported.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	TBK1 (aa514-527)
Molecular Weight	83.6
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	1-3 μg/ml
Disclaimer	For research use only

Alternative Names

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anti FLJ11330 antibody, anti NAK antibody, anti NF-kappa-B-activating kinase antibody, anti NF-kB-activating kinase antibody, anti serine/threonine-protein kinase TBK1 antibody, anti T2K antibody, anti TANK-binding kinase 1 antibody, anti TBK1 antibody

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Western blot analysis of HeLa lysate (35ug protein in RIPA buffer) using TBK1 antibody

Quick Database Links

Gene Symbol

TBK1 (aa514-527)

NCBI Gene ID

29110

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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