Human anti-SARS-CoV & CoV-2 Spike antibody (IgM), clone CR3022

SKU: orb1178881

Description

Human anti-SARS-CoV-2 Spike Antibody [CR3022] recognizes human SARS-CoV and CoV-2 Spike protein with high affinity. The binding site is amino acids 318-510 (RBD, Receptor Binding Domain) in the S1 subunit of the Spike protein. Coronavirus Spike protein conducts the process that interacts with cellular receptor and membrane fusion to allow virus entering into target cells. Spike protein also can be used to define the specificity of the virus and be used as a critical target for vaccine design. The glycosylated Spike protein can be detected in the virus-infected cell and cell culture medium. The RBD is responsible for recognizing the cell surface receptor.

Images & Validation

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Tested Applications	ELISA
Dilution range	ELISA 1:5000-20000
Reactivity	Virus

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgM
Clone No.	CR3022
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Phosphate Buffered Saline, pH 7.4.
Concentration	0.5 mg/mL
Disclaimer	For research use only

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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